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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be observed in several tumors, which leads to chromosome instability, and therefore may possibly contribute to tumorigenesis. This protein was uncovered to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have already been described. Two additional splice variants were described but in depth nucleotide sequence information is just not however out there.

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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide normally contains the epitope acknowledged because of the antibody.

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This guide outlines further doable optimization of cellular and immunoassay parameters to make sure the very best effects are...

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This information outlines even further doable optimization of mobile and immunoassay parameters to be sure the very best outcomes are...

Antibodies sure to the blocking peptide no more bind to the epitope about the target protein. By comparing the staining through the blocked antibody compared to the antibody by yourself, you can see which staining is distinct.

Goal Details Cyclin E belongs towards the highly conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins functionality as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation styles which lead to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S section boundary and is also degraded as cells progress by S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...

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